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Griseofulvin tab 125 mg /kg). Animals were treated with vehicle alone or AM281, doses of up to 5 mg/kg at 10 min intervals on test days 1–24 of each treatment week (n=25 per group). Animals on AM281 alone were tested days 1–7, day 8–14, and 15–21; AM281 with nicotine was tested on days 1–7, day 8–14, and 15–21. Data represent mean±SEM. In each test day (except 23, for which the data are from 8 animals), the 10th, 20th, and 30th% of the animals were pooled to calculate the data from days 1–10 (control animals) and 1–13 (treated according to the treatment week (Supplemental Table 1, available online) in which each animal had the experimental injection. Data were analyzed by ANOVA on the percentage of body weight gain on days 1–10 for each group. Figure 7. View largeDownload slide Adolescents treated with 3–5 mg/kg AM281 and nicotine (1 mg/kg) exhibit more nicotine-induced locomotor reactivity than controls. (A) Representative image from C57Bl/6 female mice after exposure to nicotine (1 mg/kg) (white square symbols) or drug vehicle (black triangles). Images are enhanced using a modified version of stereological method (18–20). (B) Representative image of a locomotor latency trace produced after exposure to 3 mg/kg AM281 and nicotine in C57Bl/6 mice during a 15-min latency period. The white vertical bar indicates baseline condition and each mouse was injected with vehicle (gray) or AM281 at each time point. The black bar indicates end of the latency test period and each mouse was injected with nicotine (gray) or vehicle (black) at the corresponding time points to produce a trace. (C) Representative image showing a locomotor latency trace produced after exposure to 5 mg/kg AM281 and nicotine in C57Bl/6 male mice during a 15-min latency period. The white vertical Where to buy tretinoin cream australia bar indicates baseline condition and each mouse was injected with vehicle (gray) or AM281 at each time point. The black bar indicates end of the latency test period and each mouse was injected with nicotine (gray) or vehicle (black) at the corresponding time points to produce a trace. (D) Representative image of a locomotor latency trace produced Non prescription cialis online after exposure to 6 mg/kg AM281 and nicotine in C57Bl/6 (1F10) male mice during a 15-min latency period. The white vertical bar indicates baseline condition and each mouse was injected with vehicle (gray) or AM281 at each time point. The black bar indicates end of the latency test period and each mouse was injected with nicotine (gray) or vehicle (black) at the corresponding time points to produce a trace. The locomotor latencies recorded were normalized to the vehicle injections. Animals were analyzed across multiple treatment weeks (Supplemental Table 1, available online) in which the treatment did not coincide with the behavioral testing. *significantly different from vehicle, P < 0.05. Figure 7. View largeDownload slide Adolescents fluconazole capsule price treated with 3–5 mg/kg AM281 and nicotine (1 mg/kg) exhibit more nicotine-induced locomotor reactivity than controls. (A) Representative image from C57Bl/6 female mice after exposure to nicotine (1 mg/kg) (white square symbols) or drug vehicle (black triangles). Images are enhanced using a modified version of stereological method (18–20). (B) Representative image of a locomotor latency trace produced after exposure to 3 mg/kg AM281 and nicotine in C57Bl/6 mice during a 15-min latency period. The white vertical bar indicates baseline condition and each mouse was injected with vehicle (gray) or AM281 at each time point. The black bar indicates end of the latency test period and each mouse was injected with nicotine (gray) or vehicle (black) at the corresponding fluconazol cinfa 150 mg capsulas efg time points to produce a trace. (C) Representative image showing a locomotor latency trace produced after exposure to 5 mg/kg AM281 and nicotine in C57Bl/6 male mice during a 15-min latency period. The white vertical bar indicates baseline condition and each mouse was injected with vehicle (gray) or AM281 at each time point. The black bar indicates end of the latency test period and each mouse was injected with nicotine (gray) or vehicle (black) at the corresponding time points to produce a trace. The locomotor latencies recorded were normalized to the vehicle injections. Animals were analyzed across multiple treatment weeks (Supplemental Table 1, available online) in which the treatment did not coincide with the behavioral testing. *significantly different from vehicle, P < 0.05.
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